在LTP期间, postsynaptic synaptotagmins调解AMPA受体外细胞形成过程
Dick Wu1,2,3, Taulant Bacaj1, Wade Morishita2,3
1Department of Molecular &Cellular Physiology and Howard Hughes Medical Institute, Stanford University Medical School, Stanford, California 94305, USA.
Nature
|March 30, 2017
概括
合成-1和合成-7作为冗余传感器,在长期强化 (LTP) 过程中促进AMPA受体外,这是学习和记忆的关键过程.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
背景情况:
- 长期强化 (LTP) 强化突触连接,通过NMDA受体激活和随后的流量进行中介.
- 在LTP诱导过程中的流入刺激了突触AMPA受体的招募,但潜在的机制仍然不清楚.
研究的目的:
- 为了阐明synaptotagmins在LTP期间依赖的AMPA受体招募中的作用.
主要方法:
- 研究了阻断 postsynaptic synaptotagmin-1 (Syt1) 和 synaptotagmin-7 (Syt7) 对小鼠海马CA1金字塔神经元中LTP表达的影响.
- 利用野生类型和缺乏Ca2+结合的Syt7突变,以及主导负的Syt1突变,以评估蛋白质功能.
主要成果:
- 同时阻断 postsynaptic Syt1 和 Syt7 消除了 LTP,而单独阻断任何一个都没有.
- 野生型Syt7恢复了LTP,但不是缺乏Ca2+结合的突变,这表明需要结合.
- 主导阴性Syt1突变体的表达抑制了Ca2+依赖的突触后AMPA受体表细胞和阻断了LTP.
结论:
- 后突触Syt1和Syt7在LTP期间冗余地作为AMPA受体外细胞的传感器.
- 这确定了AMPA受体招募的新机制,这对于突触可塑性,学习和记忆至关重要.
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