在T淋巴细胞激活过程中,对胰岛素敏感的甘酸酸氨基醇的调节和功能
G N Gaulton1, K L Kelly, J Pawlowski
1Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104.
Cell
|June 17, 1988
概括
T淋巴细胞合成独特的葡萄糖酸氨基醇 (gly-PI) 分子. 这些gly-PI对胰岛素敏感,可能通过释放异醇酸盐-糖来作为激素的第二信使.
科学领域:
- 细胞和分子生物学 细胞和分子生物学
- 免疫学 免疫学 免疫学
- 内分泌学 在内分泌学.
背景情况:
- 葡萄糖酸氨基醇 (gly-PI) 分子是参与细胞信号传递的复杂脂质.
- T淋巴细胞在免疫反应和细胞通信中起着至关重要的作用.
研究的目的:
- 为了分离和生化表征由T淋巴细胞合成的gly-PI分子.
- 调查糖-PI在T细胞激活和对胰岛素反应中的作用.
主要方法:
- 代谢标签与化学或酶修饰相结合.
- 单独的GLY-PI分子的生物化学特征.
- 糖-PI水解的分析和产品的识别.
主要成果:
- T淋巴细胞在线激素激活时合成独特的Gly-PI分子,具有不同的合成模式.
- 随着时间的推移,GLY-PI水平增加,与胰岛素受体的出现并行.
- 胰岛素结合快速降解的甘油-PI,释放的内醇酸甘油 (IP-glys).
- 这种新型IP-gly产品抑制了cAMP依赖的蛋白激酶活性.
结论:
- T细胞包含一组结构相关的甘氨酸-PI分子.
- 至少有一种gly-PI对胰岛素敏感,并且可以作为激素的第二信使.
- 这一途径表明了T淋巴细胞中胰岛素信号传递的新机制.
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