线粒体向纳米尺度的化物伊米达框架-90用于活细胞中的ATP成像
Jingjing Deng1, Kai Wang1,2, Ming Wang1,2
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences (CAS) , Beijing 100190, China.
Journal of the American Chemical Society
|April 8, 2017
概括
研究人员开发了纳米尺度的极性意达框架 (ZIFs) 用于在活细胞中成像线粒体ATP. 这种新的方法跟踪细胞过程中ATP的波动,如糖解和亡.
科学领域:
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
背景情况:
- 氧化伊米达框架 (ZIF) 是功能性多孔材料,具有分子传感和药物输送的潜力.
- 准线粒体等亚细胞器官对于理解细胞功能和开发向疗法至关重要.
研究的目的:
- 报告纳米级ZIF-90首次用于准线粒体和成像活细胞中的线粒体ATP动态.
- 展示基于ZIF拆卸和光染料释放的新型传感机制.
主要方法:
- 从 Zn2+ 和 imidazole-2-carboxyaldehyde 中自组装纳米规模的 ZIF-90.
- 将罗达胺B (RhB) 封装成ZIF-90,利用其光火.
- 利用ATP和ZIF-90金属节点之间的竞争性协调来触发RhB释放以进行ATP传感.
主要成果:
- 在使用ZIF-90/RhB系统的活细胞中成功成像了线粒体ATP动态.
- 在细胞糖解和亡过程中观察到ATP水平的波动.
- 证明了ZIFs在准亚细胞器官中的潜力.
结论:
- 纳米级ZIF-90可以有效地用于准线粒体和感知活细胞中的ATP.
- 这一策略为研究细胞能量代谢和相关过程提供了一种新方法.
- 这种方法为开发有针对性的细胞内疗法提供了潜力.
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The electron transport chain is a critical component of cellular respiration, occurring in the inner mitochondrial membrane. It facilitates the transfer of high-energy electrons from reduced cofactors NADH and FADH₂ to molecular oxygen, the final electron acceptor. This transfer of electrons through a series of protein complexes is tightly coupled to the translocation of protons across the membrane, generating a proton gradient essential for ATP synthesis.Electron Flow and Proton...


