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相关概念视频

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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相关实验视频

Updated: May 11, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

CRISPR-Cas:适应变化

Simon A Jackson1, Rebecca E McKenzie2, Robert D Fagerlund1

  • 1Department of Microbiology and Immunology, University of Otago, Post Office Box 56, Dunedin 9054, New Zealand.

Science (New York, N.Y.)
|April 8, 2017
PubMed
概括

CRISPR-Cas系统提供了对病毒的适应性免疫力. 最近的进展揭示了Cas1-Cas2蛋白机械如何驱动CRISPR适应,更新遗传记忆以识别新的威胁.

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Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System

Published on: June 23, 2023

相关实验视频

Last Updated: May 11, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System
11:49

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科学领域:

  • 微生物学
  • 分子生物学
  • 免疫学

背景情况:

  • Prokaryotes 面临着来自病毒和移动基因元素的持续威胁.
  • 克里斯普尔-卡斯系统是原生生物适应性免疫系统的关键.
  • 这些系统使用遗传记忆来识别和中和外来DNA/RNA.

研究的目的:

  • 审查了解CRISPR适应机制的最新进展.
  • 突出Cas1-Cas2蛋白机制在CRISPR免疫中的作用.

主要方法:

  • 关于CRISPR-Cas系统的当前科学文献的审查.
  • 控制CRISPR适应的分子机制的分析.
  • 专注于保存的Cas1-Cas2蛋白质复合体.

主要成果:

  • CRISPR适应对于更新基因记忆来应对不断变化的威胁至关重要.
  • Cas1-Cas2蛋白质复合体是各种CRISPR-Cas系统中保存和基本的组成部分.
  • 这种机制是原核生物适应性免疫反应的核心.

结论:

  • Cas1-Cas2机制对于CRISPR-Cas适应性免疫的动态更新至关重要.
  • 了解这些机制有助于我们对 prokaryotic 防御策略的了解.
  • 这次审查巩固了关于CRISPR适应的当前知识,强调了Cas1-Cas2复合体.