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通过化微混合剂控制肌肉形成

Pengpeng Bi^1,2, Andres Ramirez-Martinez^1,2, Hui Li^1,2

¹Department of Molecular Biology, Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

Science (New York, N.Y.)

|April 8, 2017

概括

科学家发现Myomixer是一种肌肉特异性, 这种蛋白质与Myomaker一起驱动肌细胞融合,

科学领域:

分子生物学
发育生物学
细胞生物学

背景情况:

骨肌肉的形成涉及到肌细胞融合到多核肌纤维.
了解肌肉融合的遗传调节对于肌肉发育研究至关重要.

研究的目的:

识别肌细胞融合和肌细胞生成所需的新基因.
描述一个新发现的肌肉特异性在骨肌肉形成中的功能.

主要方法:

全基因组集群定期间隔的简短平行列重复 (CRISPR) 功能丧失屏幕.
在肌细胞分化过程中基因表达的分析.
免疫光显微镜以确定蛋白质的定位.
细胞-细胞融合 (纤维细胞-纤维细胞和纤维细胞-肌细胞) 的功能测试.

主要成果:

发现了Myomixer,一个84氨基酸的肌肉特异性,对于肌肉细胞融合至关重要.
在肌肉细胞分化过程中,肌肉混合体的表达上调,对胚胎发生过程中骨肌肉的形成至关重要.
肌肉混合剂定位在血上,并与肌肉生成器一起促进肌肉细胞融合.
Myomixer-Myomaker复合体可以诱导非肌肉细胞的融合.

结论:

在骨肌肉发育过程中,Myomixer-Myomaker蛋白对是肌细胞融合的关键调节者.
这一发现为控制肌纤维形成的分子机制提供了新的见解.
Myomixer是理解和治疗肌肉相关疾病的潜在目标.

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