通过选择性和细胞透性血蛋白模型复合物检测和去除内源性一氧化碳
Saika Minegishi1, Aki Yumura1, Hirotsuna Miyoshi1
1Department of Molecular Chemistry and Biochemistry, Faculty of Science and Engineering, Doshisha University , Kyotanabe, Kyoto 610-0321, Japan.
Journal of the American Chemical Society
|April 8, 2017
概括
研究人员开发了一种新型工具,用于检测和去除一氧化碳 (CO), 这种清除剂,R8-hemoCD1,有效地去除细胞内的CO,有助于研究其生物功能.
科学领域:
- 生物化学
- 细胞生物学
- 化学生物学
背景情况:
- 一氧化碳 (CO) 是哺乳动物细胞在血代谢过程中产生的关键信号分子.
- 了解内源性CO的精确生物学作用需要检测和操纵其细胞内的水平.
研究的目的:
- 开发和描述新的选择性一氧化碳 (CO) 清除剂,hemoCD1和R8-hemoCD1.
- 研究这些碳化合物的生物活性特性,以检测和清除细胞系统中的内源性碳化合物.
主要方法:
- 合成超分子血蛋白模型复合体hemoCD1及其细胞透衍生物R8-hemoCD1.
- 使用hemoCD1进行光谱测定,量化细胞溶解物中的内源性CO.
- 在完整的细胞中评估R8-hemoCD1的细胞吸收和CO清除效率.
- 通过R8-hemoCD1去除CO对巨细胞反应性氧物种的产生和炎症的影响的评估.
主要成果:
- 血液CD1测定精确量化了肝细胞内源性CO,与胆红素水平相关.
- R8-hemoCD1被完整的细胞有效吸收并有效清除细胞内CO.
- 在巨细胞中通过R8-hemoCD1去除内源性CO,导致反应性氧物种显著增加,并加剧了炎症.
结论:
- 血液CD1是细胞溶解物内源性CO量化的有效工具.
- R8-hemoCD1是一种强大的细胞透性CO清除剂,具有探索CO生物功能的潜力.
- 通过R8-hemoCD1调节细胞内CO水平,揭示了它在调节氧化应激和炎症方面的作用.
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