循环的免疫细胞调解了Drosophila的系统性基于RNAi的适应性抗病毒反应
Michel Tassetto1, Mark Kunitomi1, Raul Andino1
1Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94122-2280, USA.
Cell
|April 8, 2017
概括
利用巨细胞来产生全身抗病毒免疫力. 这些细胞在外体内产生病毒衍生DNA (vDNA) 和病毒小干扰RNA (vsRNA),为原始动物提供被动保护.
科学领域:
- * 免疫学
- * 分子生物学
- * 关节动物病毒学
背景情况:
- * 多细胞生物需要细胞自主性和系统性免疫力来进行有效的抗病毒防御.
- * RNA干扰 (RNAi) 是关节动物的一个关键的抗病毒机制.
- * 系统性免疫包括向未感染的组织传递抗病毒信号.
研究的目的:
- * 研究体抗病毒免疫的机制.
- * 确定血细胞在抗病毒防御中的作用.
- * 探索昆虫抗病毒反应中的免疫记忆的潜力.
主要方法:
- * 利用中的巨细胞来研究系统性RNAi.
- * 分析了病毒衍生补充DNA (vDNA) 和病毒小干扰RNA (vsRNA) 的产生.
- * 研究了内源转体反转录酶在vDNA合成中的作用.
- * 含有来自血淋巴的vsRNA的纯化外体状囊泡.
主要成果:
- * 具有由血细胞介导的基于RNAi的全身免疫力.
- * 血细胞内化dRNA,通过内源逆转录酶产生vDNA.
- *vDNA作为二次vsRNA合成的模板,在外体内分泌.
- * 含有vsRNA的外基因对幼稚的提供被动抗病毒保护.
结论:
- 昆虫和脊椎动物一样使用免疫细胞来建立免疫记忆.
- * 稳定的vDNA对于产生体免疫力至关重要.
- * 外基因介导的vsRNAs是昆虫系统抗病毒防御的关键机制.
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