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Lung Tumor Cell Recruitment Assay
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在人类肺癌细胞中识别Alu转移
C S Lin1, D A Goldthwait, D Samols
1Department of Biochemistry, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106.
Cell
|July 15, 1988
概括
研究人员使用一种新的实验系统在人类细胞中证明了基因转移. 紫外线照射显著增强了Ecogpt (gpt) 基因的失活,揭示了Alu序列插入.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 基因转移,即DNA片段的移动,对于基因组进化至关重要.
- 了解人类细胞中的转移机制对于遗传研究至关重要.
- Ecogpt (gpt) 基因作为研究基因失活的可靠目标.
研究的目的:
- 为了证明和分析人类细胞中的遗传转移.
- 为了研究紫外线辐射对基因失活频率的影响.
- 为了描述插入的遗传元素的性质.
主要方法:
- 在含有Ecogpt (gpt) 标的A549细胞和UV照射的A549细胞之间建立了融合系统.
- 分析了来自非活化gpt基因的融合产品的子线.
- 排序插入的元素以识别它们的起源和特征.
主要成果:
- 紫外线照射在融合细胞中增加了gpt基因失活频率的100倍.
- 鉴定了一种具有完整 Alu 序列的子线,插入到 gpt 基因的编码区域.
- 插入的Alu元素与Blur8序列具有很高的相似性,差异仅为7个核酸.
- 观察到与Alu元素插入相关的5bp插入部位重复.
结论:
- 基因转移可以在人类细胞中诱导和研究.
- 紫外线辐射是基因转移事件的强有力的增强剂.
- 基因序列能够转换为功能基因,从而导致失活,并展示了遗传变异的潜在机制.
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