四种新的URNA被疹病毒编码
S I Lee1, S C Murthy, J J Trimble
1Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510.
Cell
|August 26, 1988
概括
疹病毒 saimiri 在T淋巴细胞中编码新的小核RNA (HSUR). 这些病毒URNAs形成具有独特结构和功能的核糖核蛋白,与细胞URNAs不同.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 疹病毒塞米里感染了马尔莫塞特T淋巴细胞.
- 病毒URNAs对于各种生物过程至关重要.
- 发现病毒编码的URNAs以前是未知的.
研究的目的:
- 识别和描述由疹病毒 saimiri编码的新型病毒URNA.
- 为了研究这些病毒URNAs的结构和组装到核糖核蛋白.
- 将病毒URNA与已知的细胞URNA进行比较.
主要方法:
- 从受感染的T淋巴细胞中提取和测序RNA.
- 新型RNA物种的识别和序列分析.
- 生物化学试验用于研究核糖蛋白的形成和帽结构.
- RNAase H降解以评估RNA的可访问性.
主要成果:
- 鉴定了四种疹病毒小米里编码的URNAs (HSURs).
- HSURs组合成低丰度的小核核核糖核蛋白蛋白 (snRNP),具有 Sm 决定物和 5' 三甲基瓜诺辛上限.
- HSUR序列与细胞URNAs相关,但与它们不同.
- 对HSURs的病毒基因含有独特的调节信号.
- HSUR 1和HSUR 2的5'端与多基化信号互补,可用于基配对.
结论:
- 疹病毒 saimiri编码独特的URNAs (HSURs),形成功能性的snRNP.
- 这些病毒 snRNP 与细胞对应物相比,具有独特的结构和潜在的功能性质.
- 这些发现扩大了对病毒RNA元素及其在宿主细胞操纵中的作用的理解.
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