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Updated: Mar 4, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

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基因编码的2 - 亚里尔-5 - 碳酸用于选择性蛋白质相交链接

Yulin Tian1, Marco Paolo Jacinto1, Yu Zeng2

  • 1Department of Chemistry, State University of New York at Buffalo , Buffalo, New York 14260, United States.

Journal of the American Chemical Society
|April 20, 2017
PubMed
概括

研究人员开发了新的光交叉链接器来绘制细胞中的蛋白相互作用. 在研究动态蛋白- 蛋白相互作用方面,N- 甲基pyrroletetrazole- lysine (mPyTK) 的类似物显示出高效率.

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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

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Genetic Encoding of a Non-Canonical Amino Acid for the Generation of Antibody-Drug Conjugates Through a Fast Bioorthogonal Reaction

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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