一种天然免疫性维相关蛋白质,特异于HIV-2和SIV
1Department of Cancer Biology, Harvard University School of Public Health, Boston, Massachusetts 02115.
Nature
|September 15, 1988
概括
在HIV-2和SIV的开放读取框架-x (orf-x) 在HIV-1中没有. 它的基因产品可用于体外复制,有助于区分HIV-2和HIV-1感染.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 人类免疫缺陷病毒1型 (HIV-1),HIV2型 (HIV-2) 和猿类免疫缺陷病毒 (SIV) 具有共同的基因组相似性.
- 在HIV-2和SIV中存在一个开放的读取框架,orf-x,但在HIV-1中不存在.
- 在orf-x中的序列保存表明病毒在感染性或复制性方面的潜在作用.
研究的目的:
- 调查ORF-X在SIV和HIV-2复制中的作用.
- 为了确定orf-x产品是否存在于病毒中.
- 评估ORF-X及其抗体的诊断潜力,以区分HIV-2和HIV-1感染.
主要方法:
- 在orf-x基因中引入过早停止的编码子.
- 在体外感染性和复制性测定.
- 使用HIV-2和SIV阳性血清样本和SIV阳性子血清进行抗体检测测定.
主要成果:
- 从SIVMAC和HIV-2SBL-6669的orf-x产品被发现与病毒有关.
- 通过停止编码子扰乱orf-x并没有影响病毒的感染力或in vitro复制.
- 对orf-x产品的抗体在很大一部分HIV-2和SIV阳性样本中被检测到,但在HIV-1阳性样本或对照中没有.
结论:
- 在SIVMAC的体外复制中,orf-x基因是不可缺少的.
- 艾滋病毒-2的orf-x基因产物或对它的抗体可以作为标记物来区分艾滋病毒-2和艾滋病毒-1感染.
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