cdc2 是 M 阶段特异性 ヒ斯 H1 激酶的一个组成部分:与 MPF 相同的证据
D Arion1, L Meijer, L Brizuela
1CNRS, Roscoff, France.
Cell
|October 21, 1988
概括
在介质分裂过程中,海星卵细胞中的一种特定的素H1激酶 (H1K) 被激活. 这种特定于M相的H1K含有cdc2蛋白,表明它与M相促进因子 (MPF) 是相同的.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 发育生物学 发展生物学
背景情况:
- 在真核细胞中观察到M阶段特异性素H1激酶 (H1K) 活性.
- 在海星卵细胞中,1-甲基氨酸诱导同步介质分裂,显著增加H1K活动.
研究的目的:
- 从海星卵细胞中实质性净化和表征激活的H1K.
- 为了确定激活的H1K是否与已知的细胞循环调节蛋白有关.
主要方法:
- 酶净化和活性测定.
- 使用抗体对抗裂变酵母的定量免疫血清cdc2.2.
- 分子尺寸测量色谱. 分子尺寸测量色谱.
- 使用p13-Sepharose进行结合测试.
主要成果:
- 纯化的海星H1K被丰富为34kDa的蛋白质.
- 这34kDa的蛋白质与抗酵母抗体交叉反应cdc2.2.
- 海星H1K与cdc2同类物共化,并与p13-Sepharose结合.
- H1K的激活动力学与MPF的激活动力学相匹配.
结论:
- 一个cdc2同类体是海星介质卵细胞中M阶段特定的H1K的子单元.
- 基因组H1激酶 (H1K) 和M相促进因子 (MPF) 可能是相同的实体.
- 希斯H1是类MPF的可能基质.
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02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
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