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对于小鼠嗅觉神经电路组合,多集群Pcdh多样性是必需的
George Mountoufaris1,2, Weisheng V Chen1,2, Yusuke Hirabayashi2,3
1Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
概括
来自三个基因集群的脊椎动物聚类原素 (Pcdh) 对嗅觉神经元 (OSN) 的发展至关重要. 它们的集体功能确保了 OSN 轴突在嗅觉球中的适当向和自我避开.
科学领域:
- 神经科学
- 分子生物学
- 遗传学
背景情况:
- 脊椎动物聚类原干蛋白 (Pcdh) 是由三个基因群编码的细胞表面蛋白质:Pcdhα,Pcdhβ和Pcdhγ.
- 这些蛋白质在细胞粘附和神经元连接中起作用.
研究的目的:
- 研究三种Pcdh基因集群在小鼠嗅觉神经元 (OSN) 发育中的合作功能.
- 确定Pcdh多样性的必要性,以便正确地将OSN组装到嗅球中的质体中.
主要方法:
- 在小鼠中分析Pcdh基因删除.
- 对OSN轴突植树和自我避开的评估.
- 在OSN中对Pcdh基因表达进行实验操纵.
主要成果:
- 删除单个Pcdh集群具有轻微的影响,但三集群删除导致了OSN轴突树木化和自我避开的严重缺陷.
- 在OSN中单个Pcdh基因表达阻止了球形成,这表明接触介导的排斥.
结论:
- 这三种Pcdh基因集群都合作产生OSN向的细胞表面多样性.
- 通过Pcdh进行自我回避和目标识别对于适当的嗅觉系统组装至关重要.
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