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Updated: Jun 19, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 30, 2010

来自大肠杆菌末端区域的DNA序列的识别,以阻止复制分叉

T M Hill1, A J Pelletier, M L Tecklenburg

  • 1Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.

Cell
|November 4, 1988
PubMed
概括

研究人员确定了一种特定的DNA序列,可以阻止大肠杆菌中的DNA复制分叉. 这个终端信号以依赖序列和方向的方式运行,需要Tus蛋白.

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Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...