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dpy-13:一个线虫原蛋白基因,影响身体形状

N von Mende¹, D M Bird, P S Albert

¹Division of Biological Sciences, University of Missouri, Columbia 65211.

|November 18, 1988

概括

凯诺拉布迪斯优雅的dpy-13基因,一个原蛋白家族成员,影响身体形状. 它的表征涉及转子子标记和DNA测序,揭示了它在线虫发育中的作用.

科学领域:

遗传学是一个遗传学.
分子生物学分子生物学
发育生物学发展生物学

背景情况:

在Caenorhabditis elegans dpy-13基因中发生的突变会导致"皮"的表型,其特点是短,粗的身体形状.
了解dpy-13的分子基础对于理解线虫形态和发育至关重要.

研究的目的:

为了克隆和表征野生类型的dpy-13基因.
确定dpy-13的分子性质及其与其他基因的关系.

主要方法:

用Tc1转子体标记来识别和分离dpy-13基因.
染色体步行被用来克隆野生类型的dpy-13基因,该基因位于ama-1基因的11kb处.
进行了DNA测序,以分析基因的结构和识别突变.

主要成果:

克隆了dpy-13基因,发现其长度约为1.2kb.
在转录区域的5'端附近绘制了三个转子体插入点.
DNA测序显示dpy-13属于原多基因家族,编码302氨基酸多.

结论:

该dpy-13基因编码一个原蛋白,可能参与C. elegans的结构完整性和身体形状的确定.
在dpy-13中,一个独特的146bp序列混合为1kb的mRNA,表明特定的调节或功能作用.

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