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Updated: Mar 3, 2026

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized ¹³C NMR

Published on: July 8, 2025

超极化[1-¹³C]-糖作为评估活体糖解的探针

Jae Mo Park¹, Marvin Wu², Keshav Datta

¹Advanced Imaging Research Center, University of Texas Southwestern Medical Center , Dallas, Texas 75390, United States.

Journal of the American Chemical Society

|May 4, 2017

概括

研究人员开发了一种新的分子探针,即超极化13C-糖酸盐,用于使用13C磁共振光谱的体内代谢研究. 这种探测器有效地实时跟踪糖解及其相关代谢物.

科学领域:

生物化学
医学成像
代谢工程

背景情况:

超极化13C磁共振光谱 (MRS) 可用于临床诊断的体内代谢途径监测.
进步需要新的分子探测器来研究疾病关键途径.
目前对体内代谢过程的有效调查存在局限性.

研究的目的:

合成和开发[1-13C]-糖酸盐 ([13C]-Glyc) 作为评估糖解的新型探针.
评估[13C]-Glyc对超极化13C MRS临床研究的适用性.
为了证明[13C]-Glyc在监测代谢状态中的体内应用.

主要方法:

简要的合成 [1-13C] - 糖.
用于动态核极化探测器的配方.
在老鼠肝脏模型中进行的超极化13C MRS研究.

主要成果:

[13C]-Glyc表现出高极化和长时间的旋转放松时间.
在体内研究中成功检测出标记为[13C]的酸盐和乳酸盐,这表明了糖解.
代谢物水平与动物的糖解状态相关 (禁食与食).

结论:

超极化[13C]-Glyc是一种可行的新药,用于对能量代谢的13C MRS临床研究.
这种探测器可以实时评估体内葡萄糖溶解.
该方法为生物化学研究中评估细胞内氧化还原状态提供了新的途径.

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Investigating Cardiac Metabolism in the Isolated Perfused Mouse Heart with Hyperpolarized [1-13C]Pyruvate and 13C/31P NMR Spectroscopy

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