Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

GPCR Desensitization01:12

GPCR Desensitization

8.4K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
8.4K
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

56
Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
56
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

18.4K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
18.4K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.9K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.9K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.4K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

18.7K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
18.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Development and validation of machine learning models for predicting functional outcome after low-dose alteplase in the extended time window for acute ischemic stroke.

Frontiers in neuroscience·2026
Same author

Joint effects of dyslipidemia and the platelet count on stroke risk: Longitudinal analysis via dynamic lipid stratification in the CHARLS cohort.

BMC neurology·2026
Same author

A small molecule disrupts G4-STAT1 interaction and synergizes with olaparib to drive cancer cell death.

Nucleic acids research·2026
Same author

Local heterochromatin enrichment promotes telomere clustering and PML nuclear body assembly at telomeres.

Cell reports·2026
Same author

Cerebrovascular compromise and cognitive decline driven by chronic heart failure.

Frontiers in neurology·2025
Same author

Electrical transport in tunably disordered metamaterials.

Physical review. E·2025

相关实验视频

Updated: Mar 3, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.9K

血小板衍生生增长因子受体β核心促成核酶过敏元件的重叠G-四重复和i-动因的后果可以解释突变的意外影响,并为小分子选择性向这两种结构提供机会,以降低基因表达

Robert V Brown1, Ting Wang2, Venkateshwar Reddy Chappeta1

  • 1College of Pharmacy, University of Arizona , 1703 East Mabel Street, Tucson, Arizona 85721, United States.

Journal of the American Chemical Society
|May 5, 2017
PubMed
概括
此摘要是机器生成的。

研究人员发现了针对PDGFR-β促进体的新型化合物,通过降低PDGFR-β活性来治疗急性肺损伤等疾病.

更多相关视频

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.0K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

相关实验视频

Last Updated: Mar 3, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.9K
In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

2.0K
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

科学领域:

  • 分子生物学
  • 遗传学
  • 药理学

背景情况:

  • 血小板衍生生长因子受体β (PDGFR-β) 途径是各种疾病的关键标.
  • 之前已经确定了PDGFR-β促进体中的G-四重复形成核酶过敏元件 (NHE).
  • 在这种NHE中,G四重复和i动机的结构和生物作用需要进一步研究.

研究的目的:

  • 调查PDGFR-β NHE中的G四重复和i动机的结构和生物学作用.
  • 开发针对PDGFR-β途径的新型分子策略.
  • 确定调节PDGFR-β表达和活性的化合物.

主要方法:

  • 在PDGFR-βNHE中G-四重复和i-动机形成的结构分析.
  • 使用 luciferase 递质体进行位点定向的突变发生研究.
  • 选和描述针对这些结构的小分子 (圆和二氧化碳胺).
  • 在体外测试以评估促进剂活性,基因表达,细胞增殖和迁移.
  • 在急性肺损伤的小鼠模型中进行临床前评估.

主要成果:

  • 具有GGA序列的3'-end G-四重复合体是PDGFR-β的主要抑制剂.
  • 在PDGFR-β中发生的点突变会影响G-四重复和i- motif形成的平衡.
  • 形素模拟物GSA1129可以选择性地向3'-end G-quadruplex.
  • 与PDGFR-βi基因发生相互作用.
  • GSA1129和NSC309874降低了PDGFR-β促进剂活性和转录水平.
  • GSA1129抑制PDGFRβ驱动的细胞增殖和迁移,并在体内减轻急性肺炎.

结论:

  • 这项研究阐明了3'-end G-quadruplex在PDGFR-β调控中的关键作用.
  • 对于基因表达控制至关重要的是了解G-四重复和i-动机结构之间的相互作用以及突变的影响.
  • GSA1129和NSC309874是PDGFR-β相关病理的有前途的治疗药物,包括急性肺损伤.