调节与Pol I转录相关的DDX21环
Yu-Hang Xing1, Run-Wen Yao1, Yang Zhang1
1State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.
Cell
|May 6, 2017
概括
一种新的长非编码RNA,SLERT,通过调节RNA聚合酶I转录来增强核糖体生物发生. SLERT与DDX21的相互作用会影响细胞增殖和瘤形成.
科学领域:
- 分子生物学
- 细胞生物学
- 癌症研究
背景情况:
- 通过RNA聚合酶I (Pol I) 的失调的rRNA合成是不受控制的细胞增殖和癌症的标志.
- 准确调节核糖体生物发生对于细胞生长和功能至关重要.
研究的目的:
- 识别和表征一种参与调节前核糖体RNA (前核糖体RNA) 转录的新型长非编码RNA (lncRNA).
- 阐明该 lncRNA 控制 Pol I 活动的机制及其对瘤发生的影响.
主要方法:
- 识别和功能性表征SLERT (增强前rRNA转录的snoRNA终端长非编码RNA).
- 分析SLERT在rRNA转录前,rRNA生成和删除后瘤形成中的作用.
- 通过超分辨率成像和生物化学测试,研究SLERT与DEAD-boxRNA酶DDX21的相互作用.
主要成果:
- SLERT是一个带有盒子H/ACAsnoRNA的lncRNA,对于rRNA前转录和rRNA生成至关重要.
- 删除SLERT会损害核糖体生物发生并减少瘤发生.
- SLERT与DDX21相互作用,破坏其围绕Pol I复合体的抑制环结构,从而增强转录.
结论:
- 通过调节DDX21活性,SLERT lncRNA在控制核糖体生物发生方面发挥着至关重要的作用.
- SLERT的调控机制为控制Pol I转录提供了新的见解,并为癌症提供了潜在的治疗点.
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