核细胞损失激活了酵母的下游促进体 in vivo
1Molecular Biology Institute, University of California, Los Angeles 90024.
Cell
|December 23, 1988
概括
在酵母中添加葡萄糖会导致核酶体的枯竭,从而激活下游的促进元素. 这种核细胞损失增强了基因转录启动和延长,即使没有上游激活器序列.
科学领域:
- 分子生物学分子生物学
- 酵母遗传学 酵母遗传学
- 基因规则 基因规则
背景情况:
- 核细胞在调节基因可访问性和转录方面发挥着至关重要的作用.
- 了解染色体结构如何影响基因表达对分子生物学来说至关重要.
- 酵母中的GAL促进器系统提供了一个强大的工具来控制基因表达和染色质动态.
研究的目的:
- 为了研究核酶体枯竭对酵母中下游促进元的活性的影响.
- 为了确定核细胞损失是否可以独立于正规上游调节序列激活促进体.
- 阐明核酶体枯竭在转录启动和延长中的作用.
主要方法:
- 使用在GAL促销器控制下具有基因组H4基因的酵母菌株.
- 通过添加葡萄糖诱导核细胞体枯竭.
- 评估酵母促进体 (PHO5,CYC1,GAL1) 的下游促进体元素 (TATA盒,I区域) 的激活,这些促进体与大肠杆菌 lacZ基因融合.
- 分析带有和没有上游激活器序列 (UAS) 的促进剂活性.
主要成果:
- 由葡萄糖诱导的核体枯竭激活了PHO5,CYC1和GAL1的下游促进元件.
- 不管UAS是否存在,PHO5下游元素都会被核细胞损失激活.
- 在CYC1和GAL1促进体中删除UAS元素仍然会在葡萄糖介导的核细胞损失时激活剩余的下游促进体.
- 这些发现表明,核细胞损失增强了体内转录启动和延长.
结论:
- 核细胞体枯竭是激活酵母体基因转录的一个重要因素.
- 下游促进元件可以通过核细胞损失被激活,这表明它在转录启动中发挥了直接作用.
- 识别下游促进体的蛋白质保持功能,至少部分,即使没有UAS介导的复合体形成.
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