通过传感器胺激酶传递信号的机制
Ivan Gushchin1,2, Igor Melnikov3, Vitaly Polovinkin4,2,5
1Institute of Complex Systems (ICS), ICS-6: Structural Biochemistry, Research Centre Jülich, 52425 Jülich, Germany. ivan.gushchin@phystech.edu valentin.gordeliy@ibs.fr.
Science (New York, N.Y.)
|May 20, 2017
概括
传感器胺激酶是重要的跨膜受体. 我们揭示了它们的结构机制, 展示了连接物如何触发螺旋转移和旋转,
科学领域:
- 微生物学和分子生物学
- 结构生物学
- 生物化学
背景情况:
- 跨膜 (TM) 受体,特别是传感器胺激酶,对于所有生命领域的双组件信号系统 (TCS) 是至关重要的.
- 这些基本受体中控制TM信号的详细结构机制在很大程度上仍未被阐明.
- 了解这些机制对于破译细胞沟通和开发新的治疗策略至关重要.
研究的目的:
- 阐明跨膜传感器胺激酶中信号传导的结构机制.
- 提供大肠杆菌酸盐/酸盐传感器基酶NarQ的高分辨率晶体结构
- 研究由连接体结合引起的形状变化及其对信号的影响.
主要方法:
- 使用X射线结晶学来确定NarQ的周等离子传感器,TM和细胞质HAMP域的结构.
- 对NarQ传感器的结合体和突变的无结合体状态进行了结构解析.
- 分析的重点是信号传输过程中的域移动和域间通信.
主要成果:
- 晶体结构显示了NARQTM螺旋体内的联体诱导的重新排列和像活塞的转移.
- 细胞质HAMP域表现出类似杆的运动,有效地将TM螺旋活塞运动转化为螺旋旋转.
- 这些发现为TCS中通过膜传输信号提供了详细的结构基础.
结论:
- 这项研究提供了一个全面的结构框架来理解跨膜双组件信号.
- 阐明的机制提供了关于环境信号如何在细胞膜中转化的见解.
- 这些发现为针对TCS的抗菌治疗的合理设计铺平了道路.
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