监管的 Intron 移除整合了动机状态和经验
Jason Gill1, Younshim Park1, J P McGinnis1
1Stowers Institute for Medical Research, 1000E 50(th) Street, Kansas City, MO 64110, USA; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA.
Cell
|May 20, 2017
概括
长期记忆的形成需要Drosophila Orb2A蛋白质. 它从未拼接的mRNA转化为蛋白质编码的形式是由pasilla基因调节的,整合了经验和内部状态.
科学领域:
- 神经科学
- 分子生物学
- 遗传学
背景情况:
- 记忆的持久性取决于内部状态和体验的突出性.
- 连接经验,内在状态和记忆持续时间的分子机制是未知的.
- 在Drosophila中,长期记忆需要Orb2A蛋白的特定聚合状态.
研究的目的:
- 阐明经验和内在状态影响长期记忆的分子机制.
- 确定控制记忆持久性的Orb2A蛋白水平的调节因素.
主要方法:
- 在成年大脑中分析Orb2A mRNA拼接.
- 基因查以确定参与Orb2A mRNA处理的因素.
- 研究帕西拉基因在记忆形成中的作用.
- 定位突变,以评估改变的Pasilla结合对记忆的影响.
主要成果:
- 在成年人大脑中,Drosophila Orb2A的mRNA存在于未连接的非蛋白质编码形式.
- 经验和内部状态汇聚到暂时增加拼接的蛋白质编码Orb2AmRNA.
- 飞基因 (Nova-1/2 ortholog) 调解了Orb2A的mRNA处理.
- 一种减少帕西拉结合和内核去除的特定内核突变会损害长期记忆.
结论:
- 对于长期记忆来说,Orb2A mRNA的经过帕西拉介导的处理至关重要.
- 这种处理整合了外部经验和内部状态,以调节Orb2A蛋白质的丰度.
- 这些发现揭示了基于内部和外部线索控制记忆时间的分子机制.
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