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应用系统层面的光谱成像和分析来揭示器官相互作用体

Alex M Valm¹, Sarah Cohen¹, Wesley R Legant²

¹Eunice Kennedy Shriver National Institute for Child Health and Human Development, NIH, Bethesda, Maryland 20892, USA.

|May 25, 2017

概括

这项研究绘制了活细胞中的器官相互作用,揭示了细胞功能至关重要的动态接触网络. 新的成像方法追踪多个器官, 提供细胞组织和动态的洞察力.

科学领域:

细胞生物学
系统生物学
显微镜

背景情况:

细胞在膜结合器官内分隔功能.
对于脂肪代谢等细胞过程来说, 有机细胞协调至关重要.
目前的成像限制了器官空间和时间组织的详细分析.

研究的目的:

开发和应用一种新的多光谱成像方法,用于器官互动体的系统级分析.
为了克服光蛋白成像中的光谱重叠限制.
在活细胞中绘制动态器官间接触.

主要方法:

使用共聚焦和格子光板显微镜.
开发了一个五步成像信息管道.
使用多光谱图像采集来区分多个光标签.

主要成果:

在活纤维细胞中映射器官数量,体积,速度,位置和动态接触.
六个器官 (ER,戈尔吉,溶酶体,过氧体,线粒体,脂质滴) 之间的多途相互作用的频率和局部性.
由微管和营养状况影响的可再生器官分布和接触模式.

结论:

开发的成像方法可以对有机体互动体进行详细的活细胞分析.
揭示了有机体之间的特征空间分布和动态接触网络.
这种方法在各种条件下广泛适用于研究细胞动力学.

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