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用于终身成像的毫秒级粘度探针的光复合物

Anh Thy Bui¹, Alexei Grichine², Alain Duperray²

¹Laboratoire de Chimie, École Normale Supérieure de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1 , 46 allée d'Italie, 69364 Lyon Cedex 07, France.

Journal of the American Chemical Society

|May 30, 2017

概括

使用复合物的新发光转子为测量粘度提供了一种新的方式. 这些探针在粘性环境中显著增加了量子产量和发光寿命,有助于细胞内研究.

科学领域:

生物化学
材料科学
分析化学

背景情况:

细胞内粘度对于细胞过程至关重要.
开发直接粘度测量工具对于生物研究至关重要.
光探测器提供了敏感的检测方法.

研究的目的:

开发用于直接细胞内粘度测量的新型发光转子.
研究 (III) 复合物作为粘度探针的性能.
在细胞成像中应用这些探针.

主要方法:

基于 (III) 复合物的新型发光转子的合成.
探测器对不同粘度的反应的特征.
应用时间采样终身成像显微镜进行细胞分析.

主要成果:

在粘性介质中增加量子产量和发光寿命4倍.
在0. 6-1200cP的粘度中,发光寿命在0. 23到0. 89ms之间.
在固定T24癌细胞中成功应用.

结论:

开发的 (III) 复合体作为有效的发光旋转器用于粘度传感.
毫秒发光寿命成像显微镜使细胞内粘度映射成为可能.
这项技术为研究细胞微环境提供了一种新工具.

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