在DNA纳米结构的结构动态网络中控制DNA酶的催化功能
Shan Wang1, Liang Yue1, Zohar Shpilt1
1Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Journal of the American Chemical Society
|June 20, 2017
概括
研究人员使用核酸纳米结构创建了可适应的催化系统. 这些结构性动态网络 (CDN) 响应外部触发,模仿生物系统通过调节或调低可调功能的组件.
科学领域:
- 系统化学
- 超分子化学
- 化学生物学
背景情况:
- 模仿复杂的细胞动态化学网络是系统化学的一个关键挑战.
- 宪法动态网络 (CDN) 通过自我适应环境触发器来模拟生物系统的多功能方法.
- 核酸纳米结构为构建响应性分子系统提供了强大的平台.
研究的目的:
- 使用核酸纳米结构构建由效应器触发的结构动态网络 (CDN).
- 在对外部刺激的反应中展示这些CDN中的可适应的催化特性.
- 调查CDN组成部分的上调和下调机制.
主要方法:
- 使用四个可交换的核酸组成部分 (AA',BA',AB',BB') 构建CDN.
- 使用效应分子 (燃料链,K+离子) 通过三重或G四重形成选择性稳定或破坏CDN组件的稳定.
- 使用定量凝电泳和功能测试监测网络重新配置和催化活性.
主要成果:
- 基于效应器存在的CDN组成部分的输入引导上调和下调.
- 展示了从动态重新配置的网络中产生的可适应的催化特性.
- 通过使用反触发器 (抗燃料链,皇冠以太) 成功将CDN重新配置为原始状态.
结论:
- 基于核酸的CDN可以有效地模仿具有可调节的催化功能的动态生物网络.
- 通过特定的效应器和反触发分子,可以实现对CDN组件的直角和可切换控制.
- 这项工作为设计各种应用的复杂适应性分子系统提供了基础.
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