向可见光谱发射的高化生物探测器的总体策略
Haoliang Chen1, Xiujing He1, Meihui Su1
1State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Pharmacy, and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Nankai University , Tianjin 300071, P. R. China.
Journal of the American Chemical Society
|June 28, 2017
概括
研究人员开发了一种使用二甲基 (BODIPY) 染料制造高光探针的新策略. 这种方法可以开发多功能生物探测器,用于检测活细胞中的各种分析物和成像.
科学领域:
- 有机化学
- 生物化学
- 光成像技术
背景情况:
- 开发高性探测器对于先进的生物传感和成像至关重要.
- 二甲基 (BODIPY) 染料是多功能光体,但需要增强光的策略.
- 现有的制造化探针的方法在范围和适用性方面可能受到限制.
研究的目的:
- 开发一个通用和简单的策略来创建基于BODIPY的高性探测器.
- 建立一个新的可见光谱的化机制.
- 证明这些探针对于检测各种生物分子和酶的实用性.
主要方法:
- 在BODIPY染料中引入了一种新型化机制,通过调节中位替代物的电子吸收能力.
- 合成了一组BODIPY衍生品,其排放最大值从绿色到远红色 (509,585,660 nm).
- 包含特定的触发动机来创建过氧化 (H2O2),硫化 (H2S) 和蛋白酶的生物探测器.
主要成果:
- 显示刺激触发的电子吸收能力减少,导致显著的光增强.
- 成功地将该策略应用于可见光谱中的各种BODIPY结构.
- 开发了一种针对线粒体的远红色探针,用于成像活细胞中的内源H2O2.
结论:
- 提出的策略为设计高性BODIPY生物探针提供了一种一般方法.
- 这种方法可以创建各种探测器来感知和成像各种分析物.
- 开发的探测器显示出在细胞成像和诊断中的应用潜力.
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