TERRA RNA对抗ATRX并保护端粒
Hsueh-Ping Chu1, Catherine Cifuentes-Rojas1, Barry Kesner1
1Howard Hughes Medical Institute, Boston, MA 02114, USA; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA; Department of Genetics, Harvard Medical School, Boston, MA 02114, USA.
Cell
|July 1, 2017
概括
长非编码RNA TERRA 调节基因表达并保持端粒稳定性. 它通过限制ATRX结合来对抗ATRX的基因目标,并防止端粒功能障碍.
科学领域:
- 基因组学
- 蛋白质组学
- 表观遗传学
- 核糖核酸生物学
背景情况:
- 长非编码RNAs (lncRNAs) 在基因调节中起着至关重要的作用.
- 端粒转录TERRA是一种功能不明的lncRNA.
- 了解TERRA的互动是解读其监管作用的关键.
研究的目的:
- 为了研究TERA端粒转录的功能.
- 为了确定TERRA的基因组目标和相互作用的蛋白质.
- 阐明TERRA在表观基因调节和端粒维护中的作用.
主要方法:
- 基因组和蛋白质组方法
- 确定TERRA目标地点
- 蛋白质-RNA相互作用网络分析
- 基因表达分析
- 端粒长度和稳定性测试
主要成果:
- TERRA 与端粒 (在 cis) 和基因区域 (在 trans) 结合.
- TERRA与包括ATRX在内的蛋白质网络相互作用.
- 在共享的目标基因中,TERRA和ATRX具有对抗性功能.
- 它可以抑制端粒中的ATRX结合,从而确保稳定性.
- 导致端粒病态和端粒酶活性增加.
结论:
- TERRA作为一个表观基因调节器.
- TERRA对于维持cis体中的端粒完整性至关重要.
- TERRA和ATRX构成一个调节轴,影响基因表达和端粒功能.
相关概念视频
02:41Telomeres and Telomerase
27.7K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
27.7K
02:41Telomeres and Telomerase
7.6K
7.6K
02:36DNA Damage can Stall the Cell Cycle
10.2K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.2K
02:36DNA Damage Can Stall the Cell Cycle
3.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K
02:42Transcription Attenuation in Prokaryotes
18.8K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.8K
01:29Replication in Eukaryotes
18.1K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
18.1K