在单细胞分辨率下染色体组织的细胞周期动态
Takashi Nagano1, Yaniv Lubling2, Csilla Várnai1
1Nuclear Dynamics Programme, The Babraham Insitute, Cambridge CB22 3AT, UK.
Nature
|July 7, 2017
概括
染色体在整个细胞周期中改变形状. 单细胞Hi-C揭示了染色体结构如何在细胞分裂和DNA复制过程中动态变化.
科学领域:
- 分子生物学
- 基因组学
- 细胞生物学
背景情况:
- 在细胞周期期间,甲状腺细胞染色体会发生显著的结构变化.
- 这些变化涉及密集的线粒体和解密的间相状态之间的过渡.
- 了解这些动态对于转录,基因沉默和DNA复制等过程至关重要.
研究的目的:
- 在整个细胞周期中研究单细胞水平上的染色体构造.
- 分析染色体区,TAD,绝缘和循环的细胞周期动态.
- 构建染色体的3D结构模型及其表观基因特征.
主要方法:
- 使用单细胞Hi-C (高分辨率染色体构造捕获) 来分析数千个单细胞.
- 检查了 cis 相互作用概况,以绘制细胞周期内的细胞位置.
- 生成了全基因组的三维结构模型.
主要成果:
- 发现了与细胞周期进展相关的染色体结构连续性.
- 证明了染色体区,TAD,绝缘和循环的独特细胞周期动态.
- 观察到DNA复制与分区的增加和TAD绝缘的减少有关,而循环则保持稳定.
结论:
- 在整个细胞周期中,染色体构造不是静态的,而是动态调节的.
- 单细胞Hi-C提供了细微的染色体结构,重新解释了大量的Hi-C数据.
- 揭示了具有特定表观遗传特征的染色体区的辐射结构.
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