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基因线遗传的H3K27me3在母细胞转化期间限制增强剂功能

Fides Zenk^1,2, Eva Loeser¹, Rosaria Schiavo¹

¹Department of Chromatin Regulation, Max Planck Institute of Immunobiology and Epigenetics, D-79108 Freiburg, Germany.

Science (New York, N.Y.)

|July 15, 2017

概括

母亲遗传的表观遗传标记,如H3K27me3,对于后代的发展至关重要. 这种由母体因素传播的抑制性基因素标记可以防止发育错误并确保适当的基因激活.

科学领域:

表观遗传学和发育生物学
遗传的分子机制

背景情况:

配子中的表观遗传修饰可以被遗传,影响后代的发育.
跨代表观遗传机制和后果在很大程度上是未知的.
基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因基因

研究的目的:

研究母亲遗传的表观遗传标记在早期胚胎发育中的作用.
阐明表观遗传记忆在几代人之间传播的分子机制.
了解破坏生殖系遗传信息的后果.

主要方法:

研究了Drosophila卵细胞和早期胚胎中抑制性基因素标记H3K27me3的遗传.
在H3K27me3的繁殖中研究了母体增强酶 (E(z) 的作用,这是Polycomb抑制复合体2的成分.
分析了H3K27me3遗传中断对细胞内基因标记 (H3K27ac) 和细胞内基因激活的影响.

主要成果:

*Drosophila* 卵细胞将抑制性质标记H3K27me3传递给后代.
在早期胚胎发育中,母体增强器对H3K27me3的繁殖至关重要.
基因线遗传的H3K27me3可以防止异常的H3K27ac积累和早期的基因激活,这对发育至关重要.
这种表观遗传记忆的破坏会导致胚胎死亡,

结论:

孕产妇遗传的H3K27me3,由孕产妇增殖,对早期胚胎发育至关重要.
在胚胎基因组激活过程中,这种表观遗传记忆调节增强剂活性和特定基因激活.
如果不保持生殖系遗传的表观遗传信息,就会导致发育失败.

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