类似谷氨酸受体的通道对于的化学反应和繁殖至关重要
Carlos Ortiz-Ramírez1, Erwan Michard1,2, Alexander A Simon2
1Instituto Gulbenkian de Ciência, Oeiras, 2780-156, Portugal.
Nature
|July 25, 2017
概括
谷氨酸受体通过引导精子细胞到它们的点和控制胚胎发育的基因表达来调节植物的繁殖. 这项研究揭示了这些通道在植物受精中的保留作用.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 谷氨酸受体在动物中介于神经传递.
- 在植物等非神经生物体中,谷氨酸受体的功能基本上是未知的.
- 植物氨酸受体类基因 (GLR) 参与各种过程,但突变体中强烈的表型很少见.
研究的目的:
- 研究GLR基因在基础陆地植物Physcomitrella patens中的作用.
- 阐明GLR通道在植物性繁殖中的功能.
主要方法:
- 在Physcomitrella patens中产生了GLR1和GLR2的功能丧失突变.
- 分析了突变系中的精子细胞行为和生殖成功.
- 研究GLR蛋白的离子通道特性及其对信号传递和基因表达的影响.
主要成果:
- GLR1和GLR2的突变导致精子细胞化学反应的缺陷,阻止它们到达女性生殖器官.
- GLR基因编码非选择性的透通道,调节细胞质水平.
- 为了诱导BELL1类转录因子的表达,GLR活性是必要的,这种转录因子对胚胎发育至关重要.
结论:
- 在植物繁殖过程中,GLR通道在精子化学反应和转录调节中起着至关重要的作用.
- 这些发现表明,在整个植物进化过程中,阴性繁殖的细胞间通信中,电离子类谷氨酸受体已经得到保护.
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