使用"全合一"质谱弹对蛋白质进行向检测
Chengsen Zhang1, Trevor Glaros2, Nicholas E Manicke1
1Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis , Indianapolis, Indiana 46202, United States.
Journal of the American Chemical Society
|August 1, 2017
概括
研究人员创建了一个用于快速质谱 (MS) 检测等离子体蛋白及其修饰 (PTM) 的3D打印盒. 这种创新装置简化了样品的制备和电离,
科学领域:
- 生物化学
- 分析化学
- 生物技术
背景情况:
- 精确检测血蛋白及其翻译后修饰 (PTM) 对于诊断和监测疾病至关重要.
- 目前用于PTM分析的方法往往涉及复杂的多步骤程序,限制了其速度和可访问性.
- 在临床环境中需要简化,快速和具有成本效益的针对性蛋白质组分析工具.
研究的目的:
- 开发一种新的,集成的3D打印卡片,用于精简,使用质谱测量检测等离子体蛋白及其PTM.
- 证明该弹能够有效地预先缩和直接对血等复杂生物样本的蛋白质目标进行电离.
- 验证开发的片对临床意义上的蛋白质形式的快速识别的实用性.
主要方法:
- 一个简单的3D打印弹的制造,其中包含基于抗体的丰富柱.
- 在弹内集成一种新的内置基质,用于直接电离捕获的蛋白质标.
- 使用质谱测量对人体血样本中的特定蛋白质形式进行向分析.
主要成果:
- 成功开发了一种易于使用的3D打印弹,可实现集成样本丰富和电离.
- 使用抗体丰富列从血中有效预缩目标蛋白.
- 通过MS分析实现了临床相关蛋白质异型和PTM的快速和灵敏检测.
- 用于分析血样本中的特定蛋白质形式的片的验证.
结论:
- 开发的3D打印弹通过质谱学提供了简化和快速的向血蛋白和蛋白形检测方法.
- 这项技术有可能显著提高用于临床诊断的蛋白质组分析的速度和可访问性.
- 综合设计克服了传统方法的局限性,为基于MS的蛋白质组的更广泛应用铺平了道路.
相关概念视频
01:21Tandem Mass Spectrometry
2.7K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.7K
01:33Peptide Identification Using Tandem Mass Spectrometry
8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.7K
01:19MALDI-TOF Mass Spectrometry
7.2K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.2K
01:21Mass Spectrometry: Complex Analysis
2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K
01:15High-Performance Liquid Chromatography: Types of Detectors
1.9K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.9K