通过可诱导的dCas9系统进行化学控制的表观基因组编辑
Tingjun Chen1, Dan Gao1, Roushu Zhang1
1Department of Chemistry and Chemical Biology, University of New Mexico , 300 Terrace Street NE, Albuquerque, New Mexico 87131, United States.
Journal of the American Chemical Society
|August 9, 2017
概括
我们开发了一种可诱导的新系统, 这种工具有助于理解基因激活时间和稳定性之间的直接联系.
科学领域:
- 分子生物学
- 表观遗传学
- 基因调控
背景情况:
- 在基因活性调节中,基因基因的修饰起着至关重要的作用.
- 鉴于技术上的局限性,确定特定基因素修饰和基因表达之间的直接因果关系是具有挑战性的.
研究的目的:
- 开发一种可诱导的系统,用于精确控制和研究基因组修饰.
- 调查基因乙化和基因激活之间的时间关系.
- 评估诱导的组织蛋白修饰的稳定性.
主要方法:
- 开发一个可诱导的系统,集成基于dCas9的准和化学诱导的近距离.
- 使用小分子将P300乙转移酶引入特定基因位置.
- 在基因27 (H3K27) 的基因组化.
- 对基因乙化和基因激活的时间动态分析.
主要成果:
- 在特定基因位点成功建立了向性和可诱导性基因素乙化系统.
- 阐明了H3K27乙化和随后的基因激活的精确时间顺序.
- 在细胞中表现出诱导的组织蛋白修饰的稳定性.
结论:
- 开发的诱导系统为剖析表观遗传学中的因果关系提供了强大的工具.
- 了解基因修饰的时间动态是理解基因调节的关键.
- 这项技术能够高精度地研究表观遗传机制.
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