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相关概念视频

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Synthesis and Functions of Calcitonin00:51

Synthesis and Functions of Calcitonin

Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...

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相关实验视频

Updated: May 10, 2026

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

拼接承诺决定了神经元特定的替代RNA处理在色素/CGRP基因表达中的过程.

S E Leff, R M Evans, M G Rosenfeld

    Cell
    |February 13, 1987
    PubMed
    概括
    此摘要是机器生成的。

    细胞特异性RNA处理的酸/CGRP基因产生不同的信使RNAs (mRNAs) 在神经元和甲状腺细胞. 这项研究表明,神经因素决定了CGRP mRNA生产的替代拼接和多化.

    更多相关视频

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
    08:39

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

    Published on: May 16, 2022

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
    07:14

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

    Published on: June 16, 2023

    相关实验视频

    Last Updated: May 10, 2026

    Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
    10:50

    Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

    Published on: April 24, 2021

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
    08:39

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

    Published on: May 16, 2022

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
    07:14

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

    Published on: June 16, 2023

    科学领域:

    • 分子生物学分子生物学
    • 神经科学是一个神经科学.
    • 遗传学 是一个遗传学.

    背景情况:

    • 酸/CGRP基因经历细胞特异性的替代RNA处理.
    • 这导致不同的信使RNA (mRNA):神经元中的CGRP mRNA和甲状腺C细胞中的calcitonin mRNA.
    • 替代多基和外子拼接是推动转录多样性的关键机制.

    研究的目的:

    • 调查素/CGRP基因的细胞特异性替代RNA处理背后的调控机制.
    • 为了确定负责神经元与甲状腺细胞差异性mRNA生产的因素或机制.
    • 探索拼接承诺监管因素在CGRP mRNA生成中的作用.

    主要方法:

    • 在异质细胞系统中表达野生类型和突变的素/CGRP基因.
    • 对产生的mRNA群体进行分析,以确定替代多基解和拼接的模式.
    • 神经元和甲状腺细胞表达模式之间的比较研究.

    主要成果:

    • 异质表达主要产生色素或CGRPmRNA,具体取决于细胞类型.
    • 有证据表明神经元具有特定的机械或影响替代拼接通路的因素.
    • 在特定的拼接路径和替代的多元 (A) 位点选择之间观察到相关性.

    结论:

    • 神经元可能表达一种调节因子,指导CGRP mRNA生产的替代拼接和多化.
    • 假设一个拼接承诺调节因子调节了前mRNA结构.
    • 这种调制可能会揭示产生特定于大脑的CGRP mRNA所必需的神秘拼接部位.