使用CRISPR-Cas9对猪内源性逆转录病毒进行非活化
Dong Niu1,2, Hong-Jiang Wei3,4, Lin Lin5
1eGenesis, Cambridge, MA 02139, USA.
Science (New York, N.Y.)
|August 12, 2017
概括
研究人员使用CRISPR-Cas9对猪内源逆转录病毒 (PERV) 进行了失活. 这一突破解决了临床异种移植的一个关键安全问题,即防止跨物种的病毒传播.
科学领域:
- 异种移植和病毒学
背景情况:
- 异种移植提供了器官短缺的解决方案,但面临着挑战,包括免疫相容性和猪内源逆转录病毒 (PERV) 传播的风险.
- 由于PERV有可能从猪传播到人类,因此存在严重的安全问题.
研究的目的:
- 确认PERV在人体细胞中的传染性及其水平转移.
- 为更安全的临床异种移植开发PERV失活的猪.
主要方法:
- 在人体细胞中确认PERV感染和水平传播.
- 使用CRISPR-Cas9基因编辑来禁用猪初级细胞系中的所有PERV.
- 通过体细胞核转移产生的PERV无活化猪.
主要成果:
- 证实PERV感染人类细胞,并在细胞之间横向传播.
- CRISPR-Cas9成功地使目标猪细胞系中的所有PERVs失活.
- 通过体细胞核转移成功产生了PERV无活化的猪.
结论:
- 在异种移植中,PERV 失活对于防止跨物种病毒传播至关重要.
- 成功生成PERV无活化的动物解决了临床异种移植的关键安全障碍.
相关概念视频
01:28CRISPR/Cas9 Genome Editing
2.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.1K
01:59CRISPR
58.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.2K