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相关概念视频

DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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相关实验视频

Updated: Feb 24, 2026

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis
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Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

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双ifg马赛克:多谱和组合马赛克基因功能分析的多功能方法

Samuel Pontes-Quero1, Luis Heredia1, Verónica Casquero-García1

  • 1Molecular Genetics of Angiogenesis Group, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro, 3, Madrid, E-28029, Spain.

Cell
|August 13, 2017
PubMed
概括
此摘要是机器生成的。

我们开发了一种新的方法来创建可诱导,光和功能性基因马赛克 (ifgMosaic) 鼠标模型. 这项技术可以在发育和疾病期间研究多个基因功能.

关键词:
大脑弓红糖没有罗莎26一个 VEGF血管新生这种病马赛克转基因发生

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions
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Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

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相关实验视频

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Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis
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Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

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Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions
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Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

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科学领域:

  • 遗传学
  • 发育生物学
  • 分子生物学

背景情况:

  • 基因马赛克分析对于理解生物系统中的基因功能至关重要.
  • 目前在脊椎动物中生成可诱导的功能遗传马赛克的方法由于技术挑战而受到限制.
  • 分析马赛克表型需要在同一组织内清晰标记和检测多个细胞克隆.

研究的目的:

  • 描述转基因和基因向小鼠和胚胎干细胞 (ES) 线的快速生成方法.
  • 在脊椎动物中进行可诱导,光和功能性遗传马赛克 (ifgMosaic) 分析.
  • 克服组装大型DNA结构和基因组向的技术困难.

主要方法:

  • 开发快速生成转基因小鼠和ES细胞系的方法.
  • 包含可诱导,光和功能性遗传马赛克分析的元素.
  • 使用先进的技术标记和检测多个细胞克隆.

主要成果:

  • 成功生成适合ifgMosaic分析的小鼠和ES细胞系.
  • 展示该技术的高时间和细胞分辨率能力.
  • 促进多重和组合基因功能的查询.

结论:

  • 描述的方法显著推进了脊椎动物的基因功能研究.
  • 如果gMosaic技术为剖析复杂的基因相互作用提供了强大的工具.
  • 这种方法提高了我们对器官发育和疾病机制的理解.