用于研究细胞生理学的应用的三级胺的光化学激活
Naeem Asad1, Davide Deodato1, Xin Lan2
1New York University Abu Dhabi , P.O. Box 129188, Abu Dhabi, United Arab Emirates.
Journal of the American Chemical Society
|August 15, 2017
概括
研究人员使用光移动保护组 (PPG) 开发了一种新的可光激活化合物. 这项创新使细胞生理学研究的生物活性分子能够快速有效地释放出来.
科学领域:
- 有机化学
- 摄影化学
- 细胞生物学
背景情况:
- 可移光保护组 (PPG) 是控制生物系统中生物活性分子释放的关键工具.
- 开发高效和快速起作用的PPG对于研究动态细胞过程至关重要.
- 8--7-基诺 (CyHQ) 部分具有作为新型PPG支架的潜力.
研究的目的:
- 通过将三级胺与CyHQ PPG结合来合成和表征新的可光激活化合物.
- 在光激活时评估生物活性分子释放的效率和动力学.
- 探索这些化合物在生物应用中的有用性,例如激活Cre重组酶和CRISPR-Cas9.
主要方法:
- 用CyHQ保护的三级胺的合成.
- 使用一光子和两光子激发 (1PE和2PE) 的光激活研究.
- 时间解析的光谱分析以确定光释放动力学.
主要成果:
- 使用CyHQ PPG成功创建了具有光活性的代表性三级氨基.
- 通过1PE和2PE显示出高效的生物活性分子释放,包括塔莫西芬和4-胺.
- 从单片激发 CyHQ 中观察到极快的三级胺光释放 (70 ps).
结论:
- CyHQ PPG有效地产生具有快速释放动力学的可光激活的三级胺.
- 这些化合物有望在细胞生理学,基因编辑 (CRISPR- Cas9) 和Cre重组酶激活中应用.
- 在生物研究中,CyHQ PPG为生物活性分子的空间时间控制提供了有价值的工具.
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