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Updated: Feb 24, 2026

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皮克罗米硫酶单个活性位点突变产生更有效的宏循环催化剂

Aaron A Koch, Douglas A Hansen, Vikram V Shende

  • 1Department of Chemistry and Biochemistry, University of California, Los Angeles , 607 Charles E. Young Drive East, Los Angeles, California 90095, United States.

Journal of the American Chemical Society
|August 25, 2017
PubMed
概括

研究人员设计了宏生物合成中的关键酶 (thioesterase),以克服催化瓶. 这种修改使得新型的表皮化素天然产品类型的生产成为可能.

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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