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一个完整的化器的动态分析揭示了少量化的生活方式

K Dimitri Kits¹, Christopher J Sedlacek¹, Elena V Lebedeva²

¹Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, Research Network Chemistry meets Microbiology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.

|August 29, 2017

概括

像Nitrospira inopinata这样的完整氨氧化剂 (comammox) 在寡质环境中繁荣发展. 它们的高氨基亲和力表明它们在化中起着重要作用,挑战了关于氨氧化古生物 (AOA) 的先前假设.

科学领域:

微生物学
环境科学
生物地质化学

背景情况:

化是循环中的一个关键步骤,传统上归因于分离氨氧化细菌 (AOB),古生物 (AOA) 和氧化细菌 (NOB).
在Nitrospira属内发现能够将氨转化为酸盐的完整氨氧化剂 (comammox) 需要了解它们的生态作用和竞争优势.

研究的目的:

调查comammox Nitrospira inopinata的生态利基和竞争能力.
将comammox Nitrospira的化动力学和基质亲和性与氨氧化古生物 (AOA) 的亲和性进行比较.

主要方法:

单独分离和表征一个纯的Nitrospira inopinata培养.
对N. inopinata和四种土壤/温泉AOA分离物的化动态的确定,包括基质亲和力和生长产量.
基因组分析以评估代谢潜力.

主要成果:

在低和动态息地,Nitrospira inopinata表现出适应缓慢生长,其特点是高氨基亲和度和低最大氧化率.
与常规化剂相比,N. inopinata的生长产量更高.
研究的AOA分离物显示出令人惊的低基质亲和度和较低的生长产量,表明它们可能不是最具竞争力的氨氧化剂.
除了海洋AOANitrosopumilus maritimus SCM1外,N. inopinata在分析的分离物中具有最高的基质亲和力.

结论:

康马莫克斯生物,特别是Nitrospira inopinata,非常适合在寡质和动态环境中进行化.
这些发现挑战了长期以来在寡头营养条件下AOA占主导地位的观点,并突出了comammox在这些领域的竞争潜力.

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