具有直接化学发光发射模式的近红外二氧化光源
Ori Green1, Samer Gnaim1, Rachel Blau1
1School of Chemistry, Raymond and Beverly Sackler Faculty of Exact Sciences, and ‡Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University , Tel Aviv 69978, Israel.
Journal of the American Chemical Society
|August 31, 2017
概括
研究人员开发了新的近红外 (NIR) 化学发光探针,用于直接体内成像. 这些探针能够在生物系统中灵敏地检测比如β-银酸酶和过氧化等酶.
科学领域:
- 生物医学成像
- 化学生物学
- 发光效应
背景情况:
- 化学发光探测器为检测和成像提供高灵敏度.
- 近红外 (NIR) 辐射对于体内成像至关重要,因为其可以更好地透到组织中,并且信号与噪声的比率更高.
- 目前的体内化学发光成像依赖于能量传输,限制了直接发射的能力.
研究的目的:
- 开发第一个具有直接NIR光发射的化学发光光体,用于体内应用.
- 在生理条件下创建可启动的探针来检测特定的分析物和酶.
- 证明这些探针在体外和体内生物成像中的有用性.
主要方法:
- 将扩展π电子系统的替代物纳入Schaap的阿达曼蒂利丁二氧化探针.
- 设计和合成两种新型NIR发射化学发光光体.
- 用分析剂响应组掩盖光子体,以创建β-银酸酶和过氧化的启动探针.
主要成果:
- 成功合成了两种具有直接NIR发射的新化学发光光体.
- 开发了Turn-ON探针,用于敏感地检测和成像β-银酸酶活性和过氧化.
- 在小鼠炎症模型中展示了β- 银酶活性的体外成像和体内成像.
结论:
- 开发的策略可以实现直接NIR化学发光,克服能量传输系统的局限性.
- 这些新型探测器适用于生理条件,并为非侵入性静脉成像提供潜力.
- 这项工作为使用先进的化学发光成像技术探索复杂的生物系统开辟了新的途径.
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