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Updated: Feb 23, 2026

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
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如何通过Cas1-Cas2介导间隔器集成来建立CRISPR-Cas类型II免疫力

Yibei Xiao1, Sherwin Ng1, Ki Hyun Nam2

  • 1Department of Molecular Biology and Genetics, Cornell University, 253 Biotechnology Building, Ithaca, New York 14853, USA.

Nature
|September 5, 2017
PubMed
概括

CRISPR-Cas系统使用Cas1-Cas2蛋白质将外来DNA间隔器集成到CRISPR阵列中,形成适应性免疫. 这项研究揭示了Enterococcus faecalis中间隔器集成的逐步机制,突出了DNA曲和领导近位偏好.

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