通过使用微流体桥回路通过孔隙检测离子电流的可检测尺寸范围的显著扩展
Hirotoshi Yasaki1,2, Takao Yasui1,2,3, Takeshi Yanagida4,5
1Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University , Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Journal of the American Chemical Society
|September 8, 2017
概括
这项研究引入了一种使用离子电流传感检测微小粒子的新方法. 这项技术显著提高了灵敏度,使得检测的样本体积比以前可能小得多.
科学领域:
- 生物物理
- 纳米技术
- 分析化学
背景情况:
- 通过纳米和微孔进行离子电流测量用于生物分子检测.
- 传统方法具有有限的可检测颗粒体积 (孔体积的1%),限制了复杂混合物的分析.
- 这种局限性阻碍了基于孔隙的传感对各种生物分子样本的应用.
研究的目的:
- 开发一种新的检测样本的方法,可显著减少可检测颗粒体积 (0.01%的孔径体积).
- 克服常规离子电流测量的局限性,用于分析具有广泛悬浮颗粒尺寸范围的样品.
主要方法:
- 使用微流体桥梁电路来测量由潜在差异产生的短暂电流.
- 开发了一种合理的方法来抑制背景离子电流从微安培 (μA) 到皮卡安培 (pA) 的水平.
- 使用微尺度长孔结构 (体积:5.6 × 10^4 aL) 进行增强检测.
主要成果:
- 成功检测了各种样本,包括聚乙烯纳米颗粒 (4 aL),细菌,癌细胞和DNA分子.
- 实现了背景离子电流的大量抑制,降低了可检测粒子体积极限.
- 已经证明能够检测到孔积的0.01%的颗粒.
结论:
- 拟议的方法显著扩大了离子电流传感系统的适用性.
- 这种技术适用于分析具有广泛颗粒大小的复杂生物分子样本.
- 克服了基于孔隙的各种生物样本的先前技术障碍.
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