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由Lgr5和Lgr6标记的解剖学和功能上不同的肺介质细胞群

Joo-Hyeon Lee¹, Tuomas Tammela², Matan Hofree³

¹Stem Cell Program and Divisions of Hematology/Oncology and Pulmonary & Respiratory Diseases, Boston Children's Hospital, Boston, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Wellcome Trust/Medical Research Council Stem Cell Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK; Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3DY, UK.

|September 9, 2017

概括

研究人员确定Lgr5和Lgr6是肺介质细胞的关键标志物. 这些细胞,包括光滑肌细胞,对于呼吸道和膜上皮的再生和修复至关重要.

科学领域:

肺部生物学
干细胞生物学
复原医学

背景情况:

在肺部调节上皮质平衡和再生的特定介质细胞群仍然在很大程度上没有特征.
了解这些相互作用对于开发肺部疾病的疗法至关重要.

研究的目的:

识别和描述成年肺中的新型介质细胞群.
阐明这些细胞在上皮维护和修复中的作用.

主要方法:

在小鼠中追踪基因谱系.
一个细胞RNA测序 (scRNA-seq).
有机体培养模型.

主要成果:

典型的干细胞标志物Lgr5和Lgr6在肺部识别出不同的介质细胞亚群.
Lgr6+ 细胞是与呼吸道相关的光滑肌细胞,通过 Wnt-Fgf10 信号促进呼吸道上皮分化.
Lgr5+细胞存在于膜区域,并通过Wnt激活驱动膜上皮的分化.
切除Lgr6+细胞会阻碍体内呼吸道损伤的修复.

结论:

Lgr5和Lgr6标志着功能上不同的介质细胞,对肺上皮质稳定和再生至关重要.
由Wnt信号介导的区域特异性介质-上皮交声控制了肺组织的维持.
这些发现揭示了肺部修复和再生的新细胞参与者和机制.

关键词:

没有信号膜上皮质支气管上皮差异化一个肺肺干细胞介质细胞一个小区

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