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相关概念视频

CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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The term self-esteem is often used generically, to refer to how people feel about themselves. However, according to research, there are three distinct constructs that should not be used interchangeably (Brown & Marshall, 2006). 
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When more than one gene is responsible for a given phenotype, the trait is considered polygenic. Human height is a polygenic trait. Studies have uncovered hundreds of loci that influence height, and there are believed to be many more. Due to the high number of genes involved, as well as environmental and nutritional factors, height varies significantly within a given population. The distribution of height forms a bell-shaped curve, with relatively few individuals in the population at the...
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Apart from the measures of central tendency, distribution, outliers, and the changing characteristics of data with time, an important characteristic of any data set is its variation or spread. In some data sets, the data values are concentrated closely near the mean; in others, the data values are more widely spread out from the mean.
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相关实验视频

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Improved Genome Editing via Oviductal Nucleic Acids Delivery-based In Vivo Electroporation Technique for Knockout Mice Generation
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通过基因组编辑改进作物的定量特征变异

Daniel Rodríguez-Leal1, Zachary H Lemmon1, Jarrett Man2

  • 1Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Cell
|September 19, 2017
PubMed
概括

植物促进者的CRISPR/Cas9基因组编辑为作物育种创造了新的遗传变异. 这种方法可以快速改善果实大小和植物结构等特征,提高作物产量.

科学领域:

  • 植物遗传学和育种
  • 基因组编辑技术
  • 农业科学

背景情况:

  • 提高作物产量对于未来的粮食安全至关重要.
  • 传统的植物育种依赖于基因调节区域的自然突变的缓慢选择.
  • CRISPR/Cas9基因组编辑提供了一个强大的基因改造工具.

研究的目的:

  • 证明CRISPR/Cas9促进剂编辑能够在作物中产生有益的定量特征变化.
  • 开发一种快速的方法来评估新型 cis-regulatory 的影响.
  • 通过微调产量组件来提高作物生产率.

主要方法:

  • 利用CRISPR/Cas9编辑番茄基因促进体,产生多样化的cis调节性等位基因.
  • 采用一种利用跨代Cas9活动的基因方案在异卵性功能丧失突变体中.
  • 对果实大小,花朵分支和植物结构的表型影响进行了评估.

主要成果:

  • 产生了多样化的促销者变体,为繁殖提供了有益的定量变化.
  • 成功将特定的发起人编辑与生产力特征的重大变化联系起来.
  • 在无转基因植物中快速选择和固定新型有益的等位基因.
关键词:
在CRISPR/Cas9中在 QTL种植方式关于监管的在家养开花时间果实大小鲜花花期数量变化西红

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结论:

  • 编辑CRISPR/Cas9促销器是一种有效的策略,用于在作物育种中产生新的遗传变异.
  • 开发的方法加快了对提高产量的有益等位基因的评估和选择.
  • 这种方法为了解基因调节及其在定量特征控制中的作用提供了基础.