增殖,衰老和瘤进展的β细胞在高增殖性胰腺小岛
G Teitelman1, S Alpert, D Hanahan
1Division of Neurobiology, Cornell University Medical College, New York, New York 10021.
Cell
|January 15, 1988
概括
胰腺表达氨酸氧酶 (TH) 的β细胞通常衰老,但当coprotein存在时,可以增殖并形成瘤. 这表明TH-胰岛素细胞可能逃脱衰老并成为不朽的瘤细胞.
科学领域:
- 细胞生物学 细胞生物学
- 内分泌学 在内分泌学.
- 神经科学是一个神经科学.
背景情况:
- 胰腺β细胞对于葡萄糖平衡至关重要.
- 贝塔细胞的增生可能发生在各种生理和病理条件下.
- 氨酸氧酶 (TH) 是一种神经元酶,通常与胰腺β细胞无关.
研究的目的:
- 研究表达氨酸氧酶 (TH) 的胰腺β细胞的一个子类的作用和行为.
- 了解TH表达β细胞在不同条件下的增殖能力和潜在命运,包括瘤发生.
主要方法:
- 在不同条件下的小鼠胰腺β细胞种群的比较分析:正常怀孕,肥胖和癌基因诱导的瘤.
- 细胞增殖测试可以区分"仅胰岛素"细胞和"TH-胰岛素"细胞.
- 在TH表达β细胞中观察细胞分裂和衰老标志物.
主要成果:
- 在胰腺β细胞增生病例中,观察到TH表达β细胞数量的增加.
- 在非瘤源的条件下 (怀孕,肥胖),TH-胰岛素细胞没有分裂,与"仅胰岛素"细胞不同,这表明一种通往衰老的途径.
- 型蛋白质的存在使TH-胰岛素细胞的增殖成为可能,这表明从衰老和进展到不朽的瘤细胞的潜在逃脱.
结论:
- 胰腺β细胞的一个独特的子类表达氨酸氧酶 (TH).
- 这些TH-胰岛素细胞似乎在正常生理生长和肥胖中经历衰老.
- 瘤转化可以诱导TH-胰岛素细胞的增殖,这表明瘤发育和不朽的机制.
相关概念视频
05:12Murine Nasal Lavage Fluid Collection without Blood Contamination
1.7K
The present protocol describes a novel method for avoiding blood contamination in murine nasal lavage fluid (NLF). Since this method ensures the NLF collection without blood contamination, it allows for more accurate detections of immunological components and various respiratory...
1.7K
03:40Nasolacrimal Lavage as a Treatment for Ocular Surface Toxic Soup Syndrome
1.1K
OSTSS leads to pooled inflammatory mediators in the tear film, causing symptoms like epiphora and discomfort. Here, we present a case where therapeutic nasolacrimal lavage every 2 months resolved epiphora and itchiness, suggesting its efficacy as a therapeutic intervention for OSTSS. Additionally, we report subjective improvements in symptoms in 3 additional...
1.1K
04:35Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment
880
Saline nasal lavage can be used to sample the canine nasal immune microenvironment. Because the approach is relatively non-invasive and does not disrupt the nasal tissues, it can be performed serially. Cells and proteins collected from the nasal lavage technique can be processed for various laboratory analyses.
880
08:47Symptom Assessment of Patients with Allergic Rhinitis Using an Allergen Exposure Chamber
2.9K
A protocol for conducting a challenge in an allergen exposure chamber (AEC) facility is presented. AECs have proven to be safe and effective tools for the induction of allergic symptoms or as an endpoint in the efficacy testing of allergen immunotherapy due to their ability to maintain stable particle concentrations and environmental...
2.9K
06:49Reduced Itraconazole Concentration and Durations Are Successful in Treating Batrachochytrium dendrobatidis Infection in Amphibians
12.0K
Batrachochytrium dendrobatidis infection in amphibians can be eliminated using a reduced concentration (0.0025%) and shorter duration (five minute baths for six days) of itraconazole than is generally used. Less mortality and fewer negative side effects were observed using this modified treatment...
12.0K
07:15Intranasal Administration of CNS Therapeutics to Awake Mice
61.4K
A method to intranasally administer drugs to awake mice for the purpose of targeting the brain is described. This method allows for repeat dosing over long periods using intranasal administration of drug without anesthesia, and nose-to-brain delivery with minimal systemic...
61.4K