光合作用生物的活性氧化位点的差异
Marius Retegan1, Dimitrios A Pantazis2
1European Synchrotron Radiation Facility , 71 Avenue des Martyrs, 38000 Grenoble, France.
Journal of the American Chemical Society
|September 27, 2017
概括
蓝色细菌和植物之间的光系统II的氧化复合体的差异是由于单个氨基酸的变化. 这一发现揭示了光合作用过程中水氧化和基质的原子级细节.
科学领域:
- 生物化学
- 光合作用研究
- 结构生物学
背景情况:
- 生物水氧化发生在光系统II (PSII) 的氧化复合体 (OEC).
- 不同的光谱特征和活动地点的可访问性表明OEC在不同的光合作用生物中存在结构和电子差异.
- 在PSII中,Mn4CaO5集群是水氧化的核心.
研究的目的:
- 确定蓝藻和更高的植物之间的OEC的原子水平差异.
- 阐明OEC功能的结构和电子基础.
- 了解基质到活性部位的传递机制.
主要方法:
- 对菌 (Thermosynechococcus vulcanus) 和类似菜的PSII的原子模型进行比较分析.
- 专注于Mn4CaO5集群附近的结构和电子差异.
- 研究氨基酸替代对结网络和道架构的影响.
主要成果:
- 用D1-Ala87 (较高的植物) 替代D1-Asn87是关键的区别.
- 这种替换显著改变了结,改变了与Mn4CaO5集群的水通道和残留物相互作用.
- 这些原子差异解释了特定物种的光谱性质和基质模拟相互作用.
结论:
- 单个氨基酸替代 (D1-Asn87到D1-Ala87) 是蓝藻和植物OEC结构和功能差异的主要决定因素.
- 这种差异影响了水道架构和基板输送机制.
- 这项研究将一个特定的水道分配给氧化复合体的活性部位.
更多相关视频
05:47Preparation of Polyoxometalate-based Photo-responsive Membranes for the Photo-activation of Manganese Oxide Catalysts
Published on: August 7, 2018
8.2K
08:57Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
10.5K
相关概念视频
Oxygenic Photosynthesis
835
Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate...
835
Anoxygenic Photosynthesis
1.4K
Anoxygenic photosynthesis is a phototrophic process that captures light energy to drive carbon fixation without producing molecular oxygen. Unlike oxygenic photosynthesis, which utilizes water as an electron donor and releases oxygen, anoxygenic phototrophs use alternative electron donors such as hydrogen sulfide (H₂S), elemental sulfur (S⁰), or thiosulfate (S₂O₃²⁻). This process is carried out by diverse groups of bacteria, including purple bacteria, green...
1.4K
The Z-Scheme of Electron Transport in Photosynthesis
14.2K
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
14.2K
Photosystems
7.6K
Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
7.6K
Photosystem II
79.2K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
79.2K
Photosystem I
70.4K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
70.4K
