通过生物氧化物调节表面电荷
Roy Govrin1, Itai Schlesinger1, Shani Tcherner1
1Department of Physics and the Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology , Technion City, Haifa 3200003, Israel.
Journal of the American Chemical Society
|October 4, 2017
概括
像尿素和糖醇这样的中性氧化物显著改变了表面电荷,影响了蛋白质的稳定性. 这种效应比标准模型预测的要大得多, 揭示了细胞相互作用的新见解.
科学领域:
- 生物物理
- 表面化学
- 分子相互作用
背景情况:
- 氧化物对蛋白质的稳定性和功能至关重要,通过不完全理解的机制与细胞盐和质子相互作用.
- 了解氧化物与表面的相互作用是解释细胞对环境变化的关键.
研究的目的:
- 为了研究生物氧化物 (尿素和糖醇) 对的表面电荷的影响,一个模型的表面.
- 阐明中性氧化物影响表面电荷和界面性质的机制.
主要方法:
- 使用超高分辨率频率调制原子力显微镜 (FM-AFM) 来测量表面电荷.
- 测量表面液体粘度以评估表面附近的溶解物积累/耗尽.
- 用Gouy-Chapman-Stern模型进行定量分析.
主要成果:
- 尿素 (蛋白质不稳定剂) 增加了超过50%的表面电荷 (pH等值+4),并产生了显著的表面积累.
- 糖醇 (蛋白质稳定剂) 几乎中和了二氧化表面电荷 (pH相当于-2),表面耗尽.
- 观察到的表面电荷变化是仅用介电常数变化预测的四倍.
结论:
- 对表面反应常数 (西兰醇脱质,阴离子结合) 的奥斯莫利特效应主要控制表面电荷.
- 中性氧化物对表面电荷和库伦互动有很大的影响.
- 这些发现挑战了现有的模型,并突出了奥斯莫利特在生物界面现象中的重要作用.
相关概念视频
Factors Influencing Microbial Growth: Osmolarity
1.0K
Osmolarity is the measure of solute concentration in a solution. It plays a critical role in determining water availability for organisms. Water moves across semipermeable membranes through osmosis, flowing from regions of lower solute concentration (more dilute) to regions of higher solute concentration (more concentrated).In high-solute environments, microbial cells lose water, leading to dehydration and inhibited growth. The extent to which water is available to microbes in such environments...
1.0K
Tonicity in Animals
126.7K
The tonicity of a solution determines if a cell gains or loses water in that solution. The tonicity depends on the permeability of the cell membrane for different solutes and the concentration of nonpenetrating solutes in the solution within and outside of the cell. If a semipermeable membrane hinders the passage of some solutes but allows water to follow its concentration gradient, water moves from the side with low osmolarity (i.e., less solute) to the side with higher osmolarity (i.e.,...
126.7K
Tonicity in Animals
5.7K
Tonicity describes the amount of solute in a solution. The measure of the tonicity of a solution, or the total amount of solutes dissolved in a specific amount of solution, is called its osmolarity. Three terms—hypotonic, isotonic, and hypertonic—are used to relate the osmolarity of a cell to the osmolarity of the extracellular fluid that contains the cells. In a hypotonic solution, such as tap water, the extracellular fluid has a lower concentration of solutes than the fluid inside...
5.7K
What Are Osmoregulation and Excretion?
38.4K
Organisms must keep bodily fluids at a constant temperature and pH while maintaining specific solute concentrations in order to support life functions. Osmoregulation is the process that balances solute and water levels.
38.4K
pH Regulation in Cells
7.9K
pH plays a critical role in maintaining normal cellular activities. It helps maintain the structure and function of various proteins, dictates the charge on cellular membranes, and is crucial for metabolic reactions inside the cell. Moreover, cells use the energy from the proton motive force to generate ATP.
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
Cytosolic pH
Under physiological conditions, the cytosolic pH is slightly more acidic than the extracellular pH. However, cells must prevent further acidification of their cytosol to...
7.9K
Osmoregulation in Fishes
53.5K
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
53.5K


