通过对抗PCNA环林的反感性寡氧核化物抑制细胞增殖
D Jaskulski1, J K deRiel, W E Mercer
1Department of Pathology, Temple University Medical School, Philadelphia, PA 19140.
Science (New York, N.Y.)
|June 10, 1988
概括
增殖细胞核抗原 (PCNA) 对于DNA合成和细胞分裂至关重要. 使用反感性寡度氧核酸抑制PCNA,会阻止细胞中的DNA合成和细胞分裂.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 增殖细胞核抗原 (PCNA) 是一种核蛋白.
- PCNA作为DNA聚合酶delta的辅因子起作用.
研究的目的:
- 研究PCNA在细胞DNA合成中的作用.
- 为了确定PCNA在细胞周期进展中的重要性.
主要方法:
- 利用反感性寡氧核酸来抑制指数增长的Balb/c3T3细胞中的PCNA.
- 作为对照剂,给出了一种感觉的寡度氧核酸.
主要成果:
- 反意义PCNA寡氧核酸完全抑制了DNA合成.
- 线粒分裂也被反感PCNA oligodeoxynucleotides完全抑制.
- 感觉氧核酸没有显示任何抑制作用.
结论:
- PCNA在细胞DNA合成中起着至关重要的作用.
- PCNA对于细胞循环的进展至关重要.
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