一种特定于免疫球蛋白八合体DNA基因的人类蛋白质含有功能性家庭盒子域
Cell
|October 7, 1988
概括
研究人员确定了一种人类的homeobox蛋白质,该蛋白质与酵母和Drosophila蛋白质类似地结合DNA. 这种在19号染色体上局部化的淋巴细胞受限蛋白质对理解homeobox基因功能和进化有重要意义.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 蛋白质科学 蛋白质科学
背景情况:
- 家庭盒域是一个保存的DNA结合基因,在各种物种中的蛋白质中发现,包括Drosophila家庭蛋白和酵母交配类型蛋白质.
- 功能性无特征的哺乳动物蛋白还含有homeobox域,这表明它们的作用多样化.
研究的目的:
- 描述一种含有 homeobox 域的新型淋巴细胞受限人体蛋白质.
- 为了研究这个人类homeobox蛋白的DNA结合特性和染色体定位.
主要方法:
- 序列分析以确定与已知homeobox域的氨基酸身份.
- 使用遗传映射技术进行染色体定位.
- 位点导向的突变发生,以创建一个突变蛋白质,在螺旋转螺旋动机中进行替代.
- 用DNA结合测试来评估蛋白质-DNA相互作用.
主要成果:
- 人类的homeobox基因在一个淋巴细胞受限制的蛋白质中被确定,该蛋白质具有33%的氨基酸同一性,以达成共识的homeobox序列.
- 这个基因被映射到19号染色体上,与其他人类的homeobox基因不同.
- 一种缺乏功能螺旋-转-螺旋动机的突变蛋白显示没有可检测的DNA结合.
- 人类的homeobox蛋白与酵母和Drosophila homeobox蛋白相同的DNA序列结合.
结论:
- 人类homeobox蛋白与酵母和Drosophila中的同类共享保存的DNA结合特征.
- 在19号染色体上发现的homeobox基因代表了人类homeobox基因家族的新增.
- 在homeobox域内的螺旋-转-螺旋图案对于DNA结合至关重要.
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