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相关概念视频

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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建立小鼠扩展的潜在干细胞

Jian Yang1, David J Ryan1, Wei Wang1

  • 1Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, UK.

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|October 12, 2017
PubMed
概括

研究人员从小鼠胚芽细胞和其他干细胞中制造出扩展的潜在干细胞 (EPSC). 这些EPSC可以为所有胚胎和非胚胎血统做出贡献,这是发育生物学上的重大进步.

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科学领域:

  • 发育生物学
  • 干细胞生物学
  • 表观遗传学

背景情况:

  • 胚胎干细胞 (ESC) 有助于体和生殖系,但不是胚胎外组织.
  • 胚胎外的组织,如 trofhectoderm 和原始内皮,对于胚胎发育至关重要.
  • 了解干细胞的潜力是再生医学和发育研究的关键.

研究的目的:

  • 建立和描述具有更广泛发展潜力的新型干细胞.
  • 研究这些新干细胞对所有胚胎血统的贡献.
  • 探索这些多功能干细胞的胚胎外血统的衍生.

主要方法:

  • 从八细胞芽细胞,ESC和诱导多能干细胞 (iPSC) 生成扩展的潜在干细胞 (EPSC).
  • Chimaera测试评估单个EPSC的发展贡献.
  • 在实验室中从EPSC获得热囊细胞干细胞和外胚内皮干细胞.
  • 表观基因组和单细胞转录基因组分析.

主要成果:

  • 从各种来源成功确定了EPSC,包括单个芽细胞体.
  • 单个EPSC证明了其对胚胎本身和托菲特皮系的贡献能力.
  • 从EPSC中获得真实的热血细胞干细胞和外胚内皮干细胞系.
  • EPSC 呈现出胚芽细胞特异的特征和动态 DNA 甲基组.

结论:

  • 扩大潜能干细胞在早期发育过程中具有特异性的细胞类型.
  • EPSC为研究早期哺乳动物发育和血统规范提供了强大的模型.
  • 成功生成小鼠EPSC表明在其他哺乳动物物种中建立类似细胞的可行性.