pituitary 特定的转录因子 GHF-1 是一个含有 homeobox 的蛋白质
M Bodner1, J L Castrillo, L E Theill
1Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla 92093.
Cell
|November 4, 1988
概括
增长激素因子1 (GHF-1) 是一种 pituitary 特定的转录因子,对生长激素基因表达至关重要. 研究人员分离了牛和老鼠的GHF-1cDNA,证实了它在细胞特异性基因调节中的作用.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 内分泌学 在内分泌学.
背景情况:
- 增长激素因子1 (GHF-1) 是一种 pituitary 特定的转录因子.
- GHF-1对于生长激素 (GH) 基因的细胞类型特异性表达至关重要.
研究的目的:
- 为了分离牛和老鼠GHF-1cDNA克隆.
- 描述GHF-1蛋白及其DNA结合域的特征.
主要方法:
- 从牛和大鼠垂体图书馆中分离GHF-1cDNA克隆.
- 对GHF-1cDNA进行DNA序列分析.
- 使用细菌表达的GHF-1融合蛋白的DNAase I足迹.
主要成果:
- 分离了牛和老鼠GHF-1cDNA,编码了一个33K蛋白质,与纯化的GHF-1一致.
- GHF-1序列包含一个类似于homeobox的区域.
- DNAase I 足迹检测证实了 homeobox 区域是 DNA 结合域.
- GHF-1的表达仅限于 pituitary somatotropic 血统细胞.
结论:
- GHF-1是一种 pituitary 特定的转录因子,具有 homeobox DNA 结合域.
- 限制GHF-1的表达与选择性GH基因转录相关.
- 哺乳动物的homeobox蛋白通常可以控制细胞类型特定的基因表达.
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