Mfsd2b对于红细胞和血小板中的斯芬戈辛-1-酸盐出口至关重要
Thiet M Vu1, Ayako-Nakamura Ishizu2, Juat Chin Foo3
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 5 Medical Drive, Singapore 117545.
Nature
|October 19, 2017
概括
主要促进体超级转运体2b (Mfsd2b) 被确定为红细胞和血小板中的斯芬戈辛-1-酸盐 (S1P) 的关键出口者,影响了血S1P水平和红细胞形态.
科学领域:
- 脂质信号传递
- 血液学
- 分子运输
背景情况:
- 斯芬辛-1-酸盐 (S1P) 是一种具有多种生物功能的关键信号脂质.
- 负责S1P分泌的细胞出口者,特别是来自红细胞和血小板,仍然未被确定.
- 了解S1P出口对于解读它在生理和疾病中的作用至关重要.
研究的目的:
- 确定负责从红细胞和血小板中输出斯芬戈辛-1-酸盐 (S1P) 的载体.
- 在体内研究受损S1P出口的生理后果.
- 阐明S1P分泌在红细胞健康和形态上的作用.
主要方法:
- 产生和分析Mfsd2b淘汰小鼠.
- 对红细胞,血小板和血进行全面的脂质分析.
- 生物化学测定证实Mfsd2b的S1P传输活动.
- 评估生理反应,包括过敏性休克和压力红色素形成.
主要成果:
- Mfsd2b 淘汰红细胞和血小板显示S1P物种的特定积累.
- 在生物化学测试和体内测试中证实Mfsd2b是S1P的活跃出口者.
- 在Mfsd2b绝杀小鼠中,血S1P水平降低了42 - 54%.
- 在压力下,Mfsd2b缺乏导致对过敏性休克的敏感性增加和红细胞形态的损伤.
结论:
- 孤儿运输体Mfsd2b对于从红细胞和血小板中输出氨酸-1-酸 (S1P) 是必不可少的.
- Mfsd2b占循环S1P的很大一部分,影响了系统S1P水平.
- Mfsd2b介导的S1P分泌对于保持红细胞完整性和形态学至关重要,这表明它在脂代谢中起着新的作用.
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